Measuring telomerase activity using label-free interaction analysis
ثبت نشده
چکیده
During the complex process of replication, DNA polymerase is unable to create fully double-stranded ends on daughter chromosomes. This results in a progressive shortening of the telomeric repeats, by around 50 to 200 bp per cell division in somatic cells (2). Once the telomere length reaches a critical lower limit, the cell loses its ability to replicate, entering a state of senescence that usually leads to cell death. The shortening of telomeric repeats is the principal mechanism underlying the limited life span of somatic cells and one of the paramount factors that drives the aging process.
منابع مشابه
Label-free ultrasensitive detection of telomerase activity via multiple telomeric hemin/G-quadruplex triggered polyaniline deposition and a DNA tetrahedron-structure regulated signal.
Label-free detection of telomerase activity was done by using telomeric hemin/G-quadruplex triggered polyaniline deposition, not only on themselves but also on the DNA tetrahedron-structure (DTS). DTS size has a great impact on telomerase accessibility, reactivity and detection sensitivity. The method has been used to evaluate bladder cancer development.
متن کاملBiosensor Techniques Used for Determination of Telomerase Activity in Cancer Cells
Measuring telomerase activity has proven successful for the determination of cancer in malignant somatic cells. Early conventional methods for the detection of telomerase activity include in vitro analysis via a primer extension assay, and the telomeric repeat amplification protocol (TRAP) assay. TRAP incorporates the polymerase chain reaction (PCR) step to increase the sensitivity of a given s...
متن کاملDNA-fueled molecular machine for label-free and non-enzymatic ultrasensitive detection of telomerase activity.
Herein, a non-enzymatic and label-free strategy based on DNA-fueled molecular machine was developed for ultrasensitive detection of telomerase activity in cancer cell extracts even at the single-cell level.
متن کاملColony Forming Unit Endothelial Cells Do not Exhibit Telomerase Alternative Splicing Variants and Activity
Introduction: Endothelial progenitor colony forming unit-endothelial cells (CFU-EC) were first believed to be the progenitors of endothelial cells, named endothelial progenitor cells. Further studies revealed that they are monocytes regulating vasculogenesis. The main hindrance of these cells for therapeutic purposes is their low frequency and limited replicative potentials. This study was unde...
متن کاملReal-Time Detection of Telomerase Activity in Cancer Cells using a Label-Free Electrochemical Impedimetric Biosensing Microchip.
The enzyme telomerase is present in about 85% of human cancers which makes it not only a good target for cancer treatment but also an excellent marker for cancer detection. Using a single stranded DNA probe specific for telomerase binding and reverse transcription tethered to an interdigital gold electrode array surface, the chromosome protection provided by the telomerase was replicated and fo...
متن کامل